The Core Unit CUMI is one of a total of three Core Units at the Faculty of Veterinary Medicine and enables users at the University of Leipzig as well as users from extra-university institutions and companies to identify and differentiate microorganisms using MALDI-TOF mass spectrometry.
Core Unit "Mass Spectrometric Identification and Differentiation of Microorganisms" (CUMI)
Our institute has several years of expertise in the differentiation of bacteria and fungi via MALDI-TOF MS. The database of the manufacturer (Bruker Daltonik GmbH) has been significantly expanded, especially for the following pathogen groups: Dermatophytes, Mucorales, Clostridium spec., Brachyspira spec., Rodentibacter pneumotropicus, Rodentibacter heylii and Muribacter muris. The database extensions for the murine pathogens were carried out in cooperation with Bruker Daltonik GmbH. Furthermore, the database was supplemented via a data transfer with the Robert Koch Institute. We are currently setting up the new Core Unit "Mass Spectrometric Identification and Differentiation of Microorganisms"; (CUMI). Institutions of the University of Leipzig but also extra-university institutions and companies can use the MALDI-TOF MS technology of our institute for scientific investigations at special conditions. The Core Unit is supervised by Prof. Wieland Schrödl and technical staff who have a high level of expertise.
In addition to the Core Unit CUMI, the Faculty of Veterinary Medicine has the Core Unit Flow Cytometry (CUDZ) of the Institute of Immunology, which is housed at the Institute of Virology. This offers users two latest-generation flow cytometers and a high-speed cell sorter. Additionally, the BioImaging Core Facility (BCF), supported by institutions of the Faculty of Veterinary Medicine and the Faculty of Life Sciences, provides access to equipment and software especially for confocal microscopic imaging and analysis.
Identification of microorganisms by means of MADLI-TOF MS
